Simultaneous Estimation of Phenylpropanolamine Hydrochloride and Triprolidine Hydrochloride in Pharmaceutical Preperations by Rp- Hplc Method

A simple and precise reversed phase HPLC method was developed for the stimulation estimation of phenylpropanolamine and triprolidine in tablet formulation. The method was carried out on a shimpack C8 (25 cm x 4.6mm i.d., 5μ) column with a mobile phase consisting of Acetonitrile: phosphate buffer (25mM) of pH 3.0 (35:65v/v) at a flow rate of 1.2ml/min.detection was carried out at 210 nm. The guaiphenesin was used as an internal standard. The retention time of phenylpropanolamine, triprolidine and guaiphenesin was 2.58, 6.53, and 3.84min respectively. The validation of propped method is specific, accurate, precise and linear. The linearity and range of phenylpropanolamine and triprolidine was to 5 to 15 μg/ml and 50 to 150μg/ml, respectively. The proposed method can be used for simulatious estimation of phenylpropanolamine and triprolidine in tablet formulation by HPLC method.